| Description |
A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins (PMID:11555828, PMID:14559847, PMID:12865317, PMID:15805301, PMID:15041462, PMID:18577768, PMID:19965576, PMID:20972997, PMID:10681376). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PMID:11555828, PMID:14559847, PMID:12865317, PMID:15805301, PMID:15041462, PMID:18577768, PMID:19965576, PMID:20972997, PMID:10681376). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2, as well as D-ring hydroxylated E1 and E2 at the C15-alpha and C16-alpha positions (PMID:11555828, PMID:14559847, PMID:12865317, PMID:15805301). Displays different regioselectivities for polyunsaturated fatty acids (PUFA) hydroxylation (PMID:15041462, PMID:18577768). Catalyzes the epoxidation of double bonds of certain PUFA (PMID:15041462, PMID:19965576, PMID:20972997). Converts arachidonic acid toward epoxyeicosatrienoic acid (EET) regioisomers, 8,9-, 11,12-, and 14,15-EET, that function as lipid mediators in the vascular system (PMID:20972997). Displays an absolute stereoselectivity in the epoxidation of eicosapentaenoic acid (EPA) producing the 17(R),18(S) enantiomer (PMID:15041462). May play an important role in all-trans retinoic acid biosynthesis in extrahepatic tissues. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid (PMID:10681376). May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent) (PMID:21068195). |
