Anti-DUX4 Antibody
Catalog No : IGX-43347
262.00€
188.00€
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| Description | Isoform 1: Transcription factor that is selectively and transiently expressed in cleavage-stage embryos (PMID:28459457). Binds to double-stranded DNA elements with the consensus sequence 5'-TAATCTAATCA-3' (PMID:28459457, PMID:28459454, PMID:29572508, PMID:30540931, PMID:30315230). Binds to chromatin containing histone H3 acetylated at 'Lys-27' (H3K27ac) and promotes deacetylation of H3K27ac. In parallel, binds to chromatin that lacks histone H3 acetylation at 'Lys-27' (H3K27ac) and recruits EP300 and CREBBP to promote acetylation of histone H3 at 'Lys-27' at new sites (PMID:26951377). Involved in transcriptional regulation of numerous genes, primarily as transcriptional activator, but mediates also repression of a set of target genes (PMID:17984056, PMID:27378237, PMID:26951377, PMID:28459457, PMID:28459454, PMID:29618456, PMID:30540931, PMID:29572508). Promotes expression of ZSCAN4 and KDM4E, two proteins with essential roles during early embryogenesis (PMID:27378237, PMID:26951377, PMID:28459457, PMID:29618456). Heterologous expression in cultured embryonic stem cells mediates also transcription of HERVL retrotransposons and transcripts derived from ACRO1 and HSATII satellite repeats (PMID:28459457). May activate expression of PITX1 (PMID:17984056). May regulate microRNA (miRNA) expression (PMID:24145033). Inappropriate expression can inhibit myogenesis and promote apoptosis (PMID:26951377, PMID:28935672, PMID:29618456). Isoform 2: Probably inactive as a transcriptional activator, due to the absence of the C-terminal region that is important for transcriptional activation. Can inhibit transcriptional activation mediated by isoform 1. Heterologous expression of isoform 2 has no deleterious effect on cell survival. | ||
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| Other names | Double homeobox protein 4, Double homeobox protein 10, DUX4, DUX10 | ||
| Cellular localization | Nucleus | ||
| Research area | Signal Transduction | ||
| Note | This product is for research purpose only, not for use in diagnostics or as therapeutics. | ||
| Uniprot ID | Q9UBX2 | ||
| Product name | Anti-DUX4 Antibody | ||
|---|---|---|---|
| Catalog No | IGX-43347 | ||
| Supplier’s Catalog No | IGX-43347 | ||
| Supplier | ImuGeX | ||
| Host species | Rabbit | ||
| Species reactivity | Human | ||
| Applications | ELISA, IHC | ||
| Recommended dilutions | IHC: 1:20-1:200 | ||
| Molecular weight | N/A | ||
| Conjugate | Unconjugated | ||
| Immunogen | Recombinant Human Double homeobox protein 4 protein (217-402AA) | ||
| Specificity | DUX4 antibody detects endogenous level of DUX4 protein | ||
| Storage | Store at +4°C for short term (1 week), for long term aliquote and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles, with each freeze and thaw cycle an antibody can lose half of its binding activity. | ||
|---|---|---|---|
| Clonality | Polyclonal | ||
| Isotype | IgG | ||
| Clone ID | N/A | ||
| Concentration | 1 mg/mL | ||
| Storage buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. | ||
| Formulation | Liquid | ||
| Purification | This antibody is immunogen affinity purified. | ||
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. | ||
| Reactivity life | 12 months when stored properly according to instructions (provided antibody remains sterile). | ||
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