Anti-GOLGA2 Antibody
Catalog No : IGX-41928
237.00€
175.00€
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| Description | Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane (Probable) (PMID:16489344). Required for normal protein transport from the endoplasmic reticulum to the Golgi apparatus and the cell membrane . Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis . Also plays a key role in spindle pole assembly and centrosome organization (PMID:26165940). Promotes the mitotic spindle pole assembly by activating the spindle assembly factor TPX2 to nucleate microtubules around the Golgi and capture them to couple mitotic membranes to the spindle: upon phosphorylation at the onset of mitosis, GOLGA2 interacts with importin-alpha via the nuclear localization signal region, leading to recruit importin-alpha to the Golgi membranes and liberate the spindle assembly factor TPX2 from importin-alpha. TPX2 then activates AURKA kinase and stimulates local microtubule nucleation. Upon filament assembly, nascent microtubules are further captured by GOLGA2, thus linking Golgi membranes to the spindle (PMID:19242490, PMID:26165940). Regulates the meiotic spindle pole assembly, probably via the same mechanism . Also regulates the centrosome organization (PMID:18045989, PMID:19109421). Also required for the Golgi ribbon formation and glycosylation of membrane and secretory proteins (PMID:16489344, PMID:17314401). | ||
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| Other names | Golgin subfamily A member 2, 130 kDa cis-Golgi matrix protein, GM130, GM130 autoantigen, Golgin-95, GOLGA2 | ||
| Cellular localization | Spindle Pole, Cis-Golgi Network Membrane, Peripheral Membrane, , Endoplasmic Reticulum-Golgi Intermediate Compartment Membrane | ||
| Research area | Cell Biology | ||
| Note | This product is for research purpose only, not for use in diagnostics or as therapeutics. | ||
| Uniprot ID | Q08379 | ||
| Product name | Anti-GOLGA2 Antibody | ||
|---|---|---|---|
| Catalog No | IGX-41928 | ||
| Supplier’s Catalog No | IGX-41928 | ||
| Supplier | ImuGeX | ||
| Host species | Rabbit | ||
| Species reactivity | Human | ||
| Applications | ELISA, IHC | ||
| Recommended dilutions | IHC: 1:20-1:200 | ||
| Molecular weight | N/A | ||
| Conjugate | Unconjugated | ||
| Immunogen | Recombinant Human Golgin subfamily A member 2 protein (20-300AA) | ||
| Specificity | GOLGA2 antibody detects endogenous level of GOLGA2 protein | ||
| Storage | Store at +4°C for short term (1 week), for long term aliquote and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles, with each freeze and thaw cycle an antibody can lose half of its binding activity. | ||
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| Clonality | Polyclonal | ||
| Isotype | IgG | ||
| Clone ID | N/A | ||
| Concentration | Concentration can vary between different batches. | ||
| Storage buffer | Phosphate-buffered saline (pH 7.3) containing 0.02% Sodium Azide (NaN3) and 50% Glycerol. | ||
| Formulation | Liquid | ||
| Purification | This antibody is immunogen affinity purified. | ||
| Handling | This product contains sodium azide (NaN3) as preservative, please take appropriate care when handling this product. | ||
| Reactivity life | 12 months when stored properly according to instructions (provided antibody remains sterile). | ||
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