Anti-EPM2A / Laforin Antibody
Catalog No : IGX-41902
237.00€
175.00€
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| Description | Plays an important role in preventing glycogen hyperphosphorylation and the formation of insoluble aggregates, via its activity as glycogen phosphatase, and by promoting the ubiquitination of proteins involved in glycogen metabolism via its interaction with the E3 ubiquitin ligase NHLRC1/malin. Shows strong phosphatase activity towards complex carbohydrates in vitro, avoiding glycogen hyperphosphorylation which is associated with reduced branching and formation of insoluble aggregates (PMID:16901901, PMID:23922729, PMID:26231210, PMID:25538239, PMID:25544560). Dephosphorylates phosphotyrosine and synthetic substrates, such as para-nitrophenylphosphate (pNPP), and has low activity with phosphoserine and phosphothreonine substrates (in vitro) (PMID:11001928, PMID:11220751, PMID:11739371, PMID:14532330, PMID:16971387, PMID:18617530, PMID:22036712, PMID:23922729, PMID:14722920). Has been shown to dephosphorylate MAPT . Forms a complex with NHLRC1/malin and HSP70, which suppresses the cellular toxicity of misfolded proteins by promoting their degradation through the ubiquitin-proteasome system (UPS). Acts as a scaffold protein to facilitate PPP1R3C/PTG ubiquitination by NHLRC1/malin (PMID:23922729). Also promotes proteasome-independent protein degradation through the macroautophagy pathway (PMID:20453062). Isoform 2: does not bind to glycogen (PMID:18617530). Lacks phosphatase activity and might function as a dominant-negative regulator for the phosphatase activity of isoform 1 and isoform 7 (PMID:18617530, PMID:22036712). Isoform 7: has phosphatase activity (in vitro). | ||
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| Other names | Laforin, EC 3.1.3.-, EC 3.1.3.16, EC 3.1.3.48, Glucan phosphatase, Glycogen phosphatase, Lafora PTPase, LAFPTPase, EPM2A | ||
| Cellular localization | Membrane, Cytoplasm, Endoplasmic Reticulum Membrane, Peripheral Membrane, Membrane, Nucleus, Membrane | ||
| Research area | Signal Transduction | ||
| Note | This product is for research purpose only, not for use in diagnostics or as therapeutics. | ||
| Uniprot ID | O95278 | ||
| Product name | Anti-EPM2A / Laforin Antibody | ||
|---|---|---|---|
| Catalog No | IGX-41902 | ||
| Supplier’s Catalog No | IGX-41902 | ||
| Supplier | ImuGeX | ||
| Host species | Rabbit | ||
| Species reactivity | Human | ||
| Applications | ELISA, IHC | ||
| Recommended dilutions | IHC: 1:20-1:200 | ||
| Molecular weight | N/A | ||
| Conjugate | Unconjugated | ||
| Immunogen | Recombinant Human Laforin protein (244-331AA) | ||
| Specificity | EPM2A antibody detects endogenous level of EPM2A protein | ||
| Storage | Store at +4°C for short term (1 week), for long term aliquote and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles, with each freeze and thaw cycle an antibody can lose half of its binding activity. | ||
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| Clonality | Polyclonal | ||
| Isotype | IgG | ||
| Clone ID | N/A | ||
| Concentration | Concentration can vary between different batches. | ||
| Storage buffer | Phosphate-buffered saline (pH 7.3) containing 0.02% Sodium Azide (NaN3) and 50% Glycerol. | ||
| Formulation | Liquid | ||
| Purification | This antibody is immunogen affinity purified. | ||
| Handling | This product contains sodium azide (NaN3) as preservative, please take appropriate care when handling this product. | ||
| Reactivity life | 12 months when stored properly according to instructions (provided antibody remains sterile). | ||
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