Anti-Nlrp3 Antibody
Catalog No : IGX-40355
262.00€
188.00€
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| Description | As the sensor component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens and other damage-associated signals, initiates the formation of the inflammasome polymeric complex, made of NLRP3, PYCARD and CASP1 (or possibly CASP4/CASP11). Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and secretion in the extracellular milieu (PMID:28847925, PMID:27374331). Activation of NLRP3 inflammasome is also required for HMGB1 secretion (PMID:22801494). The active cytokines and HMGB1 stimulate inflammatory responses. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death. Under resting conditions, NLRP3 is autoinhibited. NLRP3 activation stimuli include extracellular ATP, reactive oxygen species, K+ efflux, crystals of monosodium urate or cholesterol, amyloid-beta fibers, environmental or industrial particles and nanoparticles, cytosolic dsRNA, etc. However, it is unclear what constitutes the direct NLRP3 activator. Activation in presence of cytosolic dsRNA is mediated by DHX33 . Independently of inflammasome activation, regulates the differentiation of T helper 2 (Th2) cells and has a role in Th2 cell-dependent asthma and tumor growth. During Th2 differentiation, required for optimal IRF4 binding to IL4 promoter and for IRF4-dependent IL4 transcription. Binds to the consensus DNA sequence 5'-GRRGGNRGAG-3'. May also participate in the transcription of IL5, IL13, GATA3, CCR3, CCR4 and MAF (PMID:26098997). | ||
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| Other names | NACHT, LRR and PYD domains-containing protein 3, Cold autoinflammatory syndrome 1 protein homolog, Cryopyrin, Mast cell maturation-associated-inducible protein 1, PYRIN-containing APAF1-like protein 1, Nlrp3, Cias1 Mmig1 Nalp3 Pypaf1 | ||
| Cellular localization | Secreted, Endoplasmic Reticulum, Cytosol, Inflammasome, Nucleus | ||
| Research area | Immunology | ||
| Note | This product is for research purpose only, not for use in diagnostics or as therapeutics. | ||
| Uniprot ID | Q8R4B8 | ||
| Product name | Anti-Nlrp3 Antibody | ||
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| Catalog No | IGX-40355 | ||
| Supplier’s Catalog No | IGX-40355 | ||
| Supplier | ImuGeX | ||
| Host species | Rabbit | ||
| Species reactivity | Mouse | ||
| Applications | ELISA, IF | ||
| Recommended dilutions | IF: 1:50-1:200 | ||
| Molecular weight | N/A | ||
| Conjugate | Unconjugated | ||
| Immunogen | Recombinant Mouse NACHT, LRR and PYD domains-containing protein 3 protein (1-153AA) | ||
| Specificity | Nlrp3 antibody detects endogenous level of Nlrp3 protein | ||
| Storage | Store at +4°C for short term (1 week), for long term aliquote and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles, with each freeze and thaw cycle an antibody can lose half of its binding activity. | ||
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| Clonality | Polyclonal | ||
| Isotype | IgG | ||
| Clone ID | N/A | ||
| Concentration | 1 mg/mL | ||
| Storage buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. | ||
| Formulation | Liquid | ||
| Purification | This antibody is immunogen affinity purified. | ||
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. | ||
| Reactivity life | 12 months when stored properly according to instructions (provided antibody remains sterile). | ||
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