Imugex

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Categories: Biochemicals / Biochemicals

Product name			Nitrated MW Standards, Western Blot (SOD, SOD Dimer, BSA, Myosin)
Catalog No			USB-N2700-10
Supplier’s Catalog No			N2700-10
Supplier			US Biologicals
Source antigen
Reactivity			A mixture of molecular weight calibrated proteins that were nitrated with peroxynitrite: Superoxide dismutase: ~16kD; Superoxide dismutase (dimer): ~32kD; BSA: ~66kD; Myosin, muscle: ~215kD).
Cross reactivity
Applications
Molecular weight

Storage			-20°C
Other names
Grade			Highly Purified
Purity			Highly Purified
Form			Supplied in SDS-PAGE sample buffer, reduced.
Reactivity life			12 months
Note			For reserch purpose only
Description			Western Blot nitrated MW Standards for N2700-02 and N2700-13. Nitric oxide (NO) and reactive oxygen species (ROS) are important mediators to produce harmful or protective functions. NO can react with superoxide anions (O2-), yielding the toxic oxidizing agent peroxynitrite (ONOO-). Peroxynitrite induces nitration of tyrosine residues leading to changes of protein structure and function, and alterations in signaling pathways. Antibodies to nitrotyrosine have been used to monitor the status of peroxynitrite-induced modification of proteins. Applications: Suitable for use in Western Blot using the polyclonal anti-nitrotyrosine. Not sutiable for use with monoclonal anti-nitrotyrosine. Recommended Dilutions: Western Blot: 10ul per lane. Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.