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Categories: Biochemicals / Biochemicals

Product name			DNA Marker Ladder, 11 Fragments (50-1000bp), Ready to Use
Catalog No			USB-D3930-04
Supplier’s Catalog No			D3930-04
Supplier			US Biologicals
Source antigen
Reactivity
Cross reactivity
Applications
Molecular weight

Storage			-20°C
Other names
Grade			Molecular Biology Grade
Purity
Form			Supplied as a liquid in 10mM EDTA, 0.17% SDS, 0.015% bromophenol blue, 10% glycerol.
Reactivity life			12 months
Note			For reserch purpose only
Description			Contains 11 discrete fragments ranging from 50bp to 1000bp with a double intensity reference band at 500bp. Suitable for size determination of digested DNA. The marker yields the following 11 discrete fragments: 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100, 50. Quantity of DNA in band: Fragment (bp) ng/10ul 1000 91 900 66 800 61 700 51 600 43 500 70 400 28 300 23 200 30 100 22 50 15 Recommendations for Use: 1. Vortex gently just prior to use. 2. Do not heat before loading. 3. Apply 10ul of DNA Ladder per well. 4. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5ug/ml ethidium bromide for 30-40 minutes. *Note: This ladder was standardized using 10ul of DNA per lane on a 0.8cm thick, 13x15cm, 1% agarose gel rrun in TAE buffer. Storage and Stability: May be stored at -20°C For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards: All products should be handled by qualified personnel only, trained in laboratory procedures.