Zinc-alpha-2-glycoprotein, Recombinant, Human (ZAG, ZA2G, AZGP1, Zn-alpha-2-glycoprotein, Zn-alpha-2-GP, ZNGP1)
Catalog No : USB-136986
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| Product name | Zinc-alpha-2-glycoprotein, Recombinant, Human (ZAG, ZA2G, AZGP1, Zn-alpha-2-glycoprotein, Zn-alpha-2-GP, ZNGP1) | ||
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| Catalog No | USB-136986 | ||
| Supplier’s Catalog No | 136986 | ||
| Supplier | US Biologicals | ||
| Source antigen | 293 cells (HEK) | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 30.25 | ||
| Storage | -20°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~90% (RP-HPLC and SDS-PAGE) | ||
| Form | Supplied as a lyophilzed powder with no additives. Reconstotute with sterile ddH2O to ≥0.1mg/ml. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Description | Zinc-alpha-2-glycoprotein (ZAG) is found in body fluids such as serum, sweat, and seminal and breast cyst fluids. It is identical in aa sequence to tumor-derived lipid mobilizing factor (LMF), a protein associated with the dramatic loss of adipose body stores in cancer cachexia, and has been shown to stimulate lipolysis by adipocytes in vivo and in vitro. A role for ZAG has been proposed in the regulation of body weight, and age-dependent changes in genetically influenced obesity, and also it regulates melanin production by normal and malignant melanocytes. It has also recently been classified as a novel adipokine in that it is produced by both white and brown fat adipocytes and may be useful for the study of Diabetes and Obesity. ZA2G is a single, glycosylated polypeptide chain containing a total of 290 amino acids encoding (13-290). Recombinant protein corresponding to human ZA2G with 12 extra amino acids fused at the N-terminus expressed in HEK cells. Recombinant protein is identical to Swiss-Prot-P25311 (aa18-295, mature Zinc-Alpha-2-Glycoprotein). Biological Activity: Differentiated human SGBS adipocytes were incubated for 18h at two dose levels of rhZA2G - 5 and 20ug/ml. Lipolysis was quantified by measuring glycerol release into the medium using a standard protocol. Isoproterenol (10uM) and IBMX (100uM) were used as positive controls. "Con" stands for the negative control. There was a 3-fold increase in glycerol release with both doses. The increase was statistically significant at 5 µg/ml dose of rhZA2G (p<0.01) as well as in positive controls. Amino Acid Sequence: ASWSHPQFEK GSQENQDGRY SLTYIYTGLS KHVEDVPAFQ ALGSLNDLQF FRYNSKDRKS QPMGLWRQVEGMEDWKQDSQ LQKAREDIFM ETLKDIVEYY NDSNGSHVLQ GRFGCEIENN RSSGAFWKYY YDGKDYIEFNKEIPAWVPFD PAAQITKQKW EAEPVYVQRA KAYLEEECPA TLRKYLKYSK NILDRQDPPS VVVTSHQAPG EKKKLKCLAYDFYPGKIDVH WTRAGEVQEP ELRGDVLHNG NGTYQSWVVV AVPPQDTAPY SCHVQHSSLA QPLVVPWEAS Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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