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Categories: Biochemicals / Biochemicals

Product name			DNA Marker Lambda EcoRI, HindIII, 13 Fragments (125-21226bp), BioGenomics™
Catalog No			USB-D3930-23
Supplier’s Catalog No			D3930-23
Supplier			US Biologicals
Source antigen
Reactivity
Cross reactivity
Applications
Molecular weight

Storage			-20°C
Other names
Grade			Molecular Biology Grade
Purity
Form			Supplied as a liquid in 10mM Tris-HCl, pH 7.6, 1mM EDTA. 6X Loading Dye Solution L3350: Supplied as a liquid in 10mM Tris-HCl, pH 7.6, 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol, 60mM EDTA.
Reactivity life			12 months
Note			For reserch purpose only
Description			Lambda DNA was completely digested by both EcoRI and HindIII, phenol extracted, ethanol precipitated and dissolved in 10mM Tris-HCl (pH 7.6), 1mM EDTA. The marker yields the following 13 discrete fragments (in base pairs): 21226, 5148, 4973, 4268, 3530, 2027, 1904, 1584, 1375, 947, 831, 564, 125. The cohesive ends of the 12 nt cos site of bacteriophage lambda from fragments of 21226 bp and 3530 bp (indicated*) may anneal and form an additional band at 24756 bp. These fragments may be separated by heating at 65°C for 5min and then cooling on ice for 3min. Prepare DNA Marker as follows: 1ul (0.5ug) DNA marker 1ul of 6X Loading Dye Solution 4ul ddH2O 1. Vortex gently prior to use 2. Heat 5 min. @ 65°C, cool on ice 3 min. 3. Apply 6ul DNA marker per 5mm of agarose gel lane width. 4. Visualize bands with ethidium bromide staining. Storage and Stability: For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.