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Categories: Biochemicals / Biochemicals

Product name			DNA Marker 50bp Ladder, 13 Fragments (50-1000bp), BioGenomics™
Catalog No			USB-D3930-05
Supplier’s Catalog No			D3930-05
Supplier			US Biologicals
Source antigen
Reactivity
Cross reactivity
Applications
Molecular weight

Storage			-20°C
Other names
Grade			Molecular Biology Grade
Purity
Form
Reactivity life			12 months
Note			For reserch purpose only
Description			DNA Marker 50bp Ladder is designed for sizing and approximate quantification of wide range double-stranded DNA on agarose and polyacrylamide gels. The ladder is composed of 13 chromato- graphically purified individual DNA fragments (in base pairs): 1000, 900, 800, 700, 600, 500, 400, 300, 250, 200, 150, 100, 50. Note: The bright bands at 500 and 250bp serve as the reference. Concentration: 0.5mg DNA/ml Storage Buffer: 10mM Tris-HCl (pH 7.6), 1mM EDTA 6X Loading Dye Solution: Supplied as a liquid in 10mM Tris-HCl, pH 7.6, 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol, 60mM EDTA. Quality Control: Analysis of 0.5ug (1ul) of the DNA Ladder on agarose gel and 1ug (2ul)-on polyacrylamide gel by ethidium bromide staining generates 13 discrete band patterns. Quantity of DNA in Band: Fragment (bp) % ng/0.5ug DNA 1000 6 30 900 6 30 800 6 30 700 6 30 600 6 30 500 15 75 400 6 30 300 6 30 250 15 75 200 7 35 150 7 35 100 7 35 50 7 35 Total 100 500 Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.