GST, Recombinant, Schistosoma Japonicumm, aa1-218 (Glutathione S-Transferase, Sj26 Antigen)

Catalog No : USB-470232
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Product name GST, Recombinant, Schistosoma Japonicumm, aa1-218 (Glutathione S-Transferase, Sj26 Antigen)
Catalog No USB-470232
Supplier’s Catalog No 470232
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight 25.4
Storage -20°C
Other names
Grade Highly Purified
Purity ~90% (SDS-PAGE)
Form Supplied as a liquid in PBS, 10% glycerol.
Reactivity life 6 months
Note For reserch purpose only
Description GST, also known as, Glutathione S-transferase, represents a major group of detoxification enzymes. GST acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide range of compounds, including carcinogens, therapeutic drugs, and products of oxidative stress. As well as its enzymatic activities, GST may also bind toxins and function as transport protein. Because of this, an early term for GSTs was ligandin. GST was originally separated from Schistosomajaponicum but currently isolated from recombinant E.coli source. Source: Recombinant protein corresponding to aa1-218 from Schistosoma Japonicum GST, expressed in E. coli. Molecular Weight: ~25.4kD (218aa) Specific Activity: >30units/mg Unit Definition: The amount of enzyme that conjugate 1umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione/minute at pH 6.5 at 25°C. AA Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPK Endotoxin: <1EU/1ug (LAL) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.