Imugex

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Categories: Biochemicals / Biochemicals

Product name			Enhanced Green Fluorescent Protein, Recombinant, His-Tag (EGFP)
Catalog No			USB-219720
Supplier’s Catalog No			219720
Supplier			US Biologicals
Source antigen			Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight			32.7

Storage			-70°C
Other names
Grade			Highly Purified
Purity			~97% (SDS-PAGE, HPLC)
Form			Supplied as a lyophilized powder. Reconstitute with sterile PBS to 1mg/ml.
Reactivity life			12 months
Note			For reserch purpose only
Description			The recombinant EGFP is expressed and purified from transformed E. coli using a method that ensures high purity and maximal GFP fluorescence. Endotoxin has been removed, so the protein is suitable for in vivo injection or cell culture applications. The EGFP can be detected by both His-tag antibody and GFP antibody on Western Blot analysis. The excitation and emmission spectra for the recombinant EGFP is identical to GFP purified from Aequorea Victoria with Ex/Em=488/507nm. Source: Recombinant protein corresponding to EGFP, a 293aa monomer fused to 6XHis-tag on both N- and C-terminus, expressed in E. coli. Molecular Weight: ~32.7kD Endotoxin: <0.1ng/ug Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.