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Categories: Biochemicals / Biochemicals

Product name			Green Fluorescent Protein (GFP)
Catalog No			USB-168228
Supplier’s Catalog No			168228
Supplier			US Biologicals
Source antigen			E.coli
Reactivity
Cross reactivity
Applications
Molecular weight			28

Storage			-20°C
Other names
Grade			Highly Purified
Purity			Purified by DEAE chromatography.
Form			Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Reactivity life			6 months
Note			For reserch purpose only
Description			Green Fluorescent Protein (GFP) is used as a positive control when detecting GFP fusion proteins in GFP assays. Polyclonal GFP can detect GFP by ELISA (sandwich or capture) or western blot. Biotin conjugated polyclonal GFP used in a sandwich ELISA is well suited to titrate GFP in solution when used in combination with monoclonal GFP, using either form as the capture or detection. Control GFP can be used as a control in western blots. Researchers should determine optimal titers for applications. Source: Green Fluorescent Protein, expressed in E.coli. Applications: Suitable for use as a control. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.