Glutathione Transferase-Alpha, Recombinant, Human, Western Blot Positive Control (GST-alpha, GST A1-1, Glutathione S-transferase A1, GST HA Subunit 1, GST Class-alpha Member 1, GST-epsilon, GSTA1-1, GTH1)
Catalog No : USB-G8135-10B
531.05€
0.00€
Shipping cost plus VAT not included , delivery in 7-14 business days
| Product name | Glutathione Transferase-Alpha, Recombinant, Human, Western Blot Positive Control (GST-alpha, GST A1-1, Glutathione S-transferase A1, GST HA Subunit 1, GST Class-alpha Member 1, GST-epsilon, GSTA1-1, GTH1) | ||
|---|---|---|---|
| Catalog No | USB-G8135-10B | ||
| Supplier’s Catalog No | G8135-10B | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 5025 | ||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Purified | ||
| Purity | Highly Purified (~95%) | ||
| Form | Supplied as a lyophilized powder from PBS, 0.05% sodium azide. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Description | GST's are responsible for the metabolism of a broad range of xenobiotics and carcinogens. The enzyme catalyzes the reaction of glutathione with a wide variety of organic compounds to form thioethers, a reaction that is sometimes a first step in a detoxification process leading to mercapturic acid formation. Based on aa sequence similarities and antibody cross-reactivities, the mammalian cytosolic GSTs are divided into the following classes: alpha, mu, kappa, theta, pi, zeta, omega and sigma. GSTs exists as homodimers of ~25kD. GST A1-1 is mainly found in kidney, intestine, lung, and liver. GST M1-1 is restricted to liver and few other tissues. GST P1-1 has a widespread distribution in most tumor cells and tissues but it is absent in liver. The GST M1-1 is the type b allelic variant and corresponds to human GST Y. GSTA1: The alpha gene cluster consists of 5 genes GSTA1-GSTA5; the GSTA1 contains 222aa (chr 6p12), widely expressed in human tissues of liver, kidney, testis, adrenal and pancrease. GST alpha 1- 1 concentration as a marker for hepatocellular impairment, GSTA1 concentration in blood is a more specific marker than AST or ALT, and has a shorter half-life than ALT The GSTA1 shows enzyme activity towards CDNR; NBD-CI; delta 5 AD, PGE2 AND PGF2a synthase. Source: Human GST-alpha/GST A1-1 fusion protein with N-terminal His-tag (25kD), expressed in E. coli. Molecular Weight: Under native conditions, GST-alpha is homodimeric protein of ~50kD and under reducing conditions ~25kD. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 10ul/lane. Heat once prior to loading on gels. The loading volume may be adjusted if necessary. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
Product Details
© 2020 Imugex All Rights Reserved