Imugex

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Categories: Biochemicals / Biochemicals

Product name			Green Fluorescent Protein, Recombinant, Aequorea victoria (Jellyfish), His-Tag
Catalog No			USB-G8965-10F
Supplier’s Catalog No			G8965-10F
Supplier			US Biologicals
Source antigen			Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight			28.6

Storage			-70°C
Other names
Grade			Purified
Purity			≥90%
Form			Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM NaCl, 0.05% Tween 20, 50% glycerol.
Reactivity life			12 months
Note			For reserch purpose only
Description			GFP is a 27kD monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (395nm) and emits green light (508nm) in the absence of additional proteins, substrates, or co-factors. Intact, native GFP protein is required for fluorescence. The GFP chromophore results from the cyclization and oxidation of the sequence Ser65-Tyr66-Gly67. GFP fluorescence is stable, species independent, and suitable for a variety of applications. Source: Recombinant Aequorea victoria GFP, full length, with C-terminal HIS tag, expressed in E. coli . Molecular Weight: 28.6kD Absorbance: 395 and 470nm, emits green fluorescence at 509nm. Applications: Suitable for use as a quantitative control for GFP expression studies using GFP reporter vectors. It can also be used as a standardon protein gels and western immunoblots or for calibration of Fluorometers, flow cytometers and FACS machines. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.