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Categories: Biochemicals / Biochemicals

Product name			Glutathione S-Transferase, Schistosoma japonicum, Recombinant (GST)
Catalog No			USB-G8135-07
Supplier’s Catalog No			G8135-07
Supplier			US Biologicals
Source antigen			Recombinant, E. coli lysate
Reactivity
Cross reactivity
Applications
Molecular weight			26

Storage			-70°C
Other names
Grade			Highly Purified
Purity			Purified from E. coli lysate by glutathione-agarose chromatography. Single band detected on SDS-PAGE.
Form			Supplied as a liquid in 50mM Tris-HCl, pH 8.0. Sterile filtered through a 0.2um filter prior to purification.
Reactivity life			12 months
Note			For reserch purpose only
Description			The 26kD GST (glutathione S-Transferase) domain, encoded by the parasite Schistosoma japonicum, is easily purified by affinity chromatography on immobilized glutathione. For this reason, numerous prokaryotic expression vectors have been developed allowing research to fuse recombinant protein purification. GST is utilized as a fusion protein in protein expression from a range of prokaryotic expression vectors, including the pGEX family of vectors. Antibodies against GST will detect the expressed GST protein or a GST-fusion protein, and can be utilized for immuno-purification of GST-fusion proteins. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.