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Categories: Biochemicals / Biochemicals

Product name			Streptavidin, Recombinant, aa37-159, His-Tag (Streptomyces, STAV)
Catalog No			USB-137219
Supplier’s Catalog No			137219
Supplier			US Biologicals
Source antigen			Recombinant, E.coli
Reactivity
Cross reactivity
Applications
Molecular weight			15.6

Storage			-20°C
Other names
Grade			Highly Purified
Purity			~90% (SDS-PAGE)
Form			Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 0.15M NaCl, 20% glycerol.
Reactivity life			6 months
Note			For reserch purpose only
Description			Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin; the dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Source: Recombinant protein corresponding to Streptomyces streptavidin, fused to His-tag at N-terminal expressed in E.coli. Molecular Weight: ~15.6kD (148aa) (MALDI-TOF) AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMAEAGI TGTWYNQLGS TFIVTAGADG ALTGTYESAV GNAESRYVLT GRYDSAPATD GSGTALGWTV AWKNNYRNAH SATTWSGQYV GGAEARINTQ WLLTSGTTEA NAWKSTLVGH DTFTKVKP Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.