Peptidoglycan, M. luteus
Catalog No : USB-P3290
348.29€
0.00€
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| Product name | Peptidoglycan, M. luteus | ||
|---|---|---|---|
| Catalog No | USB-P3290 | ||
| Supplier’s Catalog No | P3290 | ||
| Supplier | US Biologicals | ||
| Source antigen | |||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | 2-8°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Purified | ||
| Purity | |||
| Form | |||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Description | Peptidoglycan is a polymer of glycopeptide linking N-acetyl or N-glycorylmuramic acid and peptide containing D-amino acid, and is a constituent of the cell wall in most prokaryotes. It is known that this substance is pyrogenic and initiates a variety of physiological activities such as pyrogenicity and reactivity with SLP Reagent 1 body fluid from silkworm. Source: Micrococcus luteus Appearance: Transparent or slightly white-colored suspension in water Concentration: 1ug/ml Potency: As reported Isolation method: The substrate is isolated from cultured M. luteus by treatment of hot TCA, followed by trypsin digestion. After sonication of isolated substance, the resultant substrate is suspended in sterilized distilled water. Caution: Peptidoglycan is insoluble in water and exists as a precipitate in the suspension. Upon use of the peptidoglycan suspension, the suspension should be mixed well by inversion of the vial and subsequent, vigorous vortexing. Notes: 1. SLP Reagent is an in vitro test reagent for peptidoglycan and/or (1->3)-beta-D-glucan detection, utilizing a self-defense mechanism of silkworm which consists of prophenoloxidase activation cascade and subsequent melanin formation in the body fluid. 2. The concentration of peptidoglycan in water is prepared as follows: Weigh dry peptidoglycan, followed by its suspension in sterilized distilled water at 1mg/ml concentration. Diluted 1000 x with sterile, distilled water. 3. Potency, expressed by ug/ml, is defined as reactivity to SLP reagent, with the value determined by use of our original peptidoglycan preparation as a reference material. | ||
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