MTHFD1, Recombinant, Pongo abelii, aa1-305, His-SUMO-Tag (C-1-tetrahydrofolate Synthase, Cytoplasmic Domain)

Catalog No : USB-374306
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Product name MTHFD1, Recombinant, Pongo abelii, aa1-305, His-SUMO-Tag (C-1-tetrahydrofolate Synthase, Cytoplasmic Domain)
Catalog No USB-374306
Supplier’s Catalog No 374306
Supplier US Biologicals
Source antigen Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight 49
Storage -20°C
Other names
Grade Highly Purified
Purity ~90% (SDS-PAGE)
Form Supplied as a liquid in Tris, 50% glycerol.
Reactivity life 6 months
Note For reserch purpose only
Purity ~90% (SDS-PAGE)
Description 5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH. 5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate. ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. Source: Recombinant protein corresponding to aa1-305 from pongo abelii MTHFD1, fused to His-SUMO-Tag at N-terminal expressed in E. coli. Molecular Weight: ~49.0kD AA Sequence: MAPAEILNGREISAQIRARLKNQVTQLKEQVPGFTPGLAILLVGNRDDSNLYINVKLKAAEEIGIKATHIKLPRTTTESEVIKYITSLNEDSTVHGFLVQLPLDSENSINTEEVINAIAPEKDVDGLTSISAGKLARGDLNDCFIPCTPKGCLELIKETGVPIAGRHAVVVGRSKIVGAPMHDLLLWNNATVTTCHSKTANLDEEVNKGDILVVATGRPEMVKGEWIKPGAIVIDCGINYVPDDKKPNGRKVVGDVAYDEAKERASFITPVPGGVGPMTVAMLMQSTVESAKRFLEKFKPGKWMI Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.